Virus-inhibitory activity of the antigen complex of opportunistic pathogenic bacteria against SARS-CoV-2

Introduction. The antigen complex of opportunistic pathogenic bacteria (ACOPB) has a protective effect against avian influenza viruses, herpes virus type 2, and other viruses that cause acute respiratory viral infections. In the context COVID-19 pandemic, an important task is to find out whether ACOPB SARS-CoV-2.
 purpose study was evaluate in vitro virus-inhibitory activity Dubrovka laboratory strain Materials methods. performed using Vero cell line CCL-81, human peripheral blood mononuclear cells (PBMCs), mouse monoclonal anti-idiotypic antibodies structurally mimicking biological effects interferons (IFNs), SARS-CoV-2. infectivity assessed by two methods: titration cultures limiting dilution method when results are cytopathic effect; second plaque assay. inhibition test culture susceptible SARS-CoV-2; mixture containing specific dose two-fold transferred after medication had interacted with at 4C for 2 hours. SARS-CoV-2 functional / IFN receptors (RIFN) PBMCs induced RIFN expression level measured indirect membrane immunofluorescence test.
 Results. Hemagglutination assay chicken, mouse, guinea pig, red detection inhibitory protein. lysate CCL-81 infected demonstrated highest hemagglutination pig low titers virus-containing fluid. inhibited 10 doses titer 1 : 32, providing 100% protection 8 days (the monitoring period). on membranes decreased its interaction Dubrovka.
 Conclusion. experimental studies including IFN-like properties both immunomodulatory effect.